Site-specific deletion and rearrangement of integron insert genes catalyzed by the integron DNA integrase
نویسندگان
چکیده
منابع مشابه
Site-specific deletion and rearrangement of integron insert genes catalyzed by the integron DNA integrase.
Deletion of individual antibiotic resistance genes found within the variable region of integrons is demonstrated. Evidence for gene duplications and rearrangements resulting from the insertion of gene units at new locations is also presented. Deletion, duplication, and rearrangement occur only in the presence of the integron-encoded DNA integrase. These events are precise and involve loss or ga...
متن کاملIntegron integrase binds to bulged hairpin DNA.
Gene cassettes are short, monogenic DNA elements that translocate between integrons through site-specific excision and integration. These events require that an integron-coded tyrosine recombinase forms a reactive complex with two sites, at least one of which belongs to the attC class. An attC site can be divided into two pairs of short repeats flanking a palindromic central region. The nucleot...
متن کاملCharacterisation of specific and secondary recombination sites recognised by the integron DNA integrase.
Integrons determine a site-specific recombination system which is responsible for the acquisition of genes, particularly antibiotic resistance genes. The integrase encoded by integrons recognises two distinct classes of recombination sites. The first is the family of imperfect inverted repeats, known as 59-base elements, which are associated with the mobile gene cassettes. The second consists o...
متن کاملA hot spot in plasmid F for site-specific recombination mediated by Tn21 integron integrase.
Integron In2 integrase (IntI1)-mediated site-specific recombination between two primary sites occurs at a high frequency, while that between a primary and a secondary site occurs at frequencies around 10,000 times lower. Secondary sites consist of a pentanucleotide with only two fully conserved residues (GWTMW). The analysis of IntI1-mediated recombinants in the plasmid pOX38 revealed the exist...
متن کاملThe IntI1 integron integrase preferentially binds single-stranded DNA of the attC site.
IntI1 integrase is a member of the prokaryotic DNA integrase superfamily. It is responsible for mobility of antibiotic resistance cassettes found in integrons. IntI1 protein, as well as IntI1-COOH, a truncated form containing its carboxy-terminal domain, has been purified. Electrophoretic mobility shift assays were carried out to study the ability of IntI1 to bind the integrase primary target s...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1992
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.174.5.1574-1585.1992